Immunohistochemical Expression of Osterix Appearing in the Mouse Orthodontic Periodontal Tension Sides

DOI Web Site 24 References
  • Harada Toshihisa
    Clinical Evaluation Unit, Matsumoto Dental University Graduate School of Oral Medicine
  • Nakano Keisuke
    Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine Department of Oral Pathology, Matsumoto Dental University School of Dentistry
  • Matsuda Hirokazu
    Department of Orthodontics, Matsumoto Dental University School of Dentistry
  • Muraoka Rina
    Department of Orthodontics, Matsumoto Dental University School of Dentistry
  • Tomoda Maki
    Department of Orthodontics, Matsumoto Dental University School of Dentistry
  • Yokoi Yukiko
    Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry
  • Yamada Kazuhiro
    Clinical Evaluation Unit, Matsumoto Dental University Graduate School of Oral Medicine Department of Orthodontics, Matsumoto Dental University School of Dentistry
  • Okafuji Norimasa
    Clinical Evaluation Unit, Matsumoto Dental University Graduate School of Oral Medicine

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Abstract

Early changes of Osterix expression were examined by immunohistochemistry (IHC) and fluorescent immunohistochemistry (FIHC) in mouse periodontal ligament exposed to experimentally induced orthodontic mechanical stress. Eight-week-old ddY mice were used as experimental animals. To provide continuous orthodontic mechanical stress on the periodontal ligament, a rubber dam sheet was placed between the upper molars of the mice. At 20 min, 1 h, 3 h, 9 h and 24 h after the insertion, related parts of the animal tissues were evaluated by IHC and FIHC regarding Osterix and Runx2 expressions. In the 3-h and 9-h experimental groups, orthodontic tension sites reacted positively. The positive reactions became weak in the experimental group at 24-h. The results suggest that Osterix acts as one of the key factors of osteogenic cell differentiation, which expresses the following after the expression of Runx2, down-steam of Runx2.

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