The Ability of Transplanted Bone Marrow-Derived Cells to Differentiate into Parenchymal Cells of Salivary Glands

• Yuan Yao-Wei

  Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine Department of Histology and Embryology, School of Basic Medical Sciences, Harbin Medical University Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Tamamura Ryo

  Department of Histology, Nihon University School of Dentistry at Matsudo

• Lei Lei

  Department of Histology and Embryology, School of Basic Medical Sciences, Harbin Medical University

• Katase Naoki

  Department of Molecular and Developmental Biology, Kawasaki Medical School

• Ara Sathi Gulsan

  Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Ito Satoshi

  Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Tsujigiwa Hidetsugu

  Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Nagatsuka Hitoshi

  Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

Abstract

Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.

Journal

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 22 (4), 433-438, 2013

  THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY

Keywords

• Bone marrow-derived cells
• Salivary gland
• Differentiation
• Parenchymal cells
• Green fluorescent protein

Details

• CRID: 1390001204459539840
• NII Article ID: 130004480613
• NII Book ID: AA11074332
• DOI: 10.2485/jhtb.22.433
• ISSN: 1880828X
• ISSN: 13417649
• NDL BIB ID: 024971702
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024971702
• Web Site: https://search.jamas.or.jp/link/ui/2015046925
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed