Establishment of Experimental Periapical Inflammatory Lesions in Mice

• Osuga Naoto

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Matsuda Saeka

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine

• Shoumura Masahito

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Moriyama Keita

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Yokoi Yukiko

  Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry

• Nakano Keisuke

  Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine Department of Oral Pathology, Matsumoto Dental University School of Dentistry

• Kawakami Toshiyuki

  Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine

Abstract

Although studies on the formation of apical periodontitis have somehow been carried out but detailed cellular dynamics remain unclear. We recently established an experiment that could easily be performed using ddY mice. First, under general anesthesia using isoflurane inhalation, the coronal portion of the maxillary first molar was penetrated using a round bur and drill with water irrigation causing pulp exposure until the root apex. Micro computed tomography (R_mCT) was taken over time during observation. Four weeks later, R_mCT confirmed the presence of a radiolucent image at the apex of the tooth, which was then removed for histological examination. The results showed that granulation tissue with fibrosis had gradually formed at the periphery of the abscess. The present method confirmed the effectiveness of the experimental mode to exmine the formation of chronic inflammatory lesions at the root apex.

Journal

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 22 (4), 517-520, 2013

  THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY

Keywords

• Inflammatory apical periodontitis
• Experimental model
• Mouse
• Micro-CT
• Histopathology

Details

• CRID: 1390282679436132736
• NII Article ID: 130004480626
• NII Book ID: AA11074332
• DOI: 10.2485/jhtb.22.517
• ISSN: 1880828X
• ISSN: 13417649
• NDL BIB ID: 024972000
• Web Site: https://mdu.repo.nii.ac.jp/records/2782
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024972000
• Web Site: https://search.jamas.or.jp/link/ui/2015046938
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed