Role of Nucleotide Excision Repair or Base Excision Repair in Movement of Various n-Alkylated Bases, Investigated by the Comet Assay

• Odajima Chihiro

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology

• Nakamura Takanori

  Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University

• Nakamura Maki

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology BoZo Research Center

• Miura Masanori

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology

• Yamasaki Kayoko

  Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University

• Honda Gisho

  Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University

• Kikuchi Yasuaki

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology

• Yamamoto Ayumi

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology

• Sasaki Yu F.

  Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University

書誌事項

タイトル別名

• Role of Nucleotide Excision Repair or Base Excision Repair in Movement of Various <i>n</i>-Alkylated Bases, Investigated by the Comet Assay

抄録

Although nucleotide excision repair (NER) removes bulky adducts upon exposure to various chemicals, base excision repair (BER) removes altered bases that cause relatively minor disturbances to the helical DNA structure. We have shown that the action of BER can be distinguished from that of NER by the enhancement of a comet-positive response by 2′,3′-dideoxythymidine (ddT). Furthermore, in this study, the action of NER is shown to be distinguishable from that of BER by the reduction of a comet-positive response by α-amanitin (AMN). To study how bulky alkylated bases can be removed by NER or BER, we studied the reduction of a comet-positive response by AMN and its enhancement by ddT for n-alkyl methanesulfonates having an n-alkyl group with 1-7 carbons. For this purpose, n-alkyl methanesulfonates having an n-alkyl group with 3-7 carbons (n-propyl methanesulfonate, n-butyl methanesulfonate, n-pentyl methanesulfonate, n-hexyl methanesulfonate, and heptyl methanesulfonate) were synthesized. AMN reduced the comet-positive response for n-alkyl methanesulfonates having an n-alkyl group with ≧4 carbons and ddT enhanced it for n-alkyl methanesulfonates having an n-alkyl group with ≦5 carbons. Therefore, it is considered that NER acts to remove bases alkylated by n-alkyl groups with ≧4 carbons, BER acts to remove bases alkylated by n-alkyl groups with ≦5 carbons, and both NER and BER act to remove bases alkylated by n-alkyl groups with 4 and 5 carbons.<br>

収録刊行物

• Genes and Environment

  Genes and Environment 36 (1), 10-16, 2014

  日本環境変異原学会

キーワード

• formation of comet tails
• NER
• BER
• ddT
• AMN

詳細情報

• CRID: 1390282680234154496
• NII論文ID: 130004481210
• NII書誌ID: AA1212552X
• DOI: 10.3123/jemsge.2013.012
• ISSN: 18807062
• ISSN: 18807046
• NDL書誌ID: 025316264
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025316264
• Web Site: https://search.jamas.or.jp/link/ui/2015047642
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可