In-situ Observation of DNA Hybridization in Aqueous Solution by Multiple Internal Reflection Infrared Absorption Spectroscopy

• MIYAMOTO Ko-ichiro

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University

• ISHIBASHI Ken-ichi

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University

• YAMAGUCHI Ryo-taro

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University

• KIMURA Yasuo

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University CREST, Japan Science and Technology Corporation

• ISHII Hisao

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University CREST, Japan Science and Technology Corporation

• NIWANO Michio

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University CREST, Japan Science and Technology Corporation

Bibliographic Information

Other Title

• 多重内部反射型赤外吸収分光法によるＤＮＡハイブリダイゼーションの液中その場観察
• タジュウ ナイブ ハンシャガタ セキガイ キュウシュウ ブンコウホウ ニ ヨル DNA ハイブリダイゼーション ノ エキ チュウ ソノバ カンサツ

Abstract

We have observed in-situ the hybridization (double helix formation) and denaturation (separation of double helix at elevated temperatures) of DNA in aqueous solution using infrared absorption spectroscopy (IRAS) in the multiple internal reflection (MIR) geometry. We demonstrated that conformational changes of DNA strands due to hybridization and denaturation are reflected in the infrared absorption spectra of the bases of DNA. Comparing with the results of ab-initio cluster calculation, we found that hybridization produces the specific C=O stretching vibration modes in the hydrogen-bonded bases, and also that the C=O stretching vibration modes of the bases of a single strand may be greatly influenced by its surrounding water molecules that interact with the bases.

Journal

• Hyomen Kagaku

  Hyomen Kagaku 26 (9), 553-558, 2005

  The Surface Science Society of Japan

Keywords

• General Computer Science

Details

• CRID: 1390001206459286528
• NII Article ID: 130004486290
• NII Article ID: 40006907373
• NII Book ID: AN00334149
• DOI: 10.1380/jsssj.26.553
• COI: 1:CAS:528:DC%2BD2MXht1ensr%2FE
• ISSN: 18814743
• ISSN: 03885321
• NDL BIB ID: 7478921
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7478921
• Web Site: http://www.jstage.jst.go.jp/article/jsssj/26/9/26_9_553/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed