Current Topics of Immunohistochemical and Biological Properties of Human Lymphatic Endothelial Cells
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- Kawai Yoshiko
- Department of Physiology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
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- Ohhashi Toshio
- Department of Physiology, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
抄録
We examined the immunohistochemical properties of selective lymph vessel markers such as LYVE-1, podoplanin, Prox-1, and VEGF R3, as well as NO synthase (NOS) and cyclo-oxygenase (COX) in two kinds of human lymphatic endothelial cell isolated from collecting and initial lymph vessels. The constitutively expressed genes in the two kinds of lymphatic endothelial cell were also evaluated using oligonucleotide microarray analysis and RT-PCR. We also investigated the effects of the oxygen concentration in culture conditions on the proliferative activities of the two kinds of human lymphatic endothelial cell. Immunoreactivity to LYVE-1 and the RT-PCR expression level of LYVE-1 mRNA in endothelial cells of initial lymph vessels were stronger than those of collecting lymph vessels. Immunoreactivity to ecNOS, iNOS, COX1, and COX2 was also found to be significantly higher than in collecting lymph vessels. In contrast, an increase in the O2 concentration ranging from 5% to 21% caused a significant reduction in the proliferative activity of endothelial cells in collecting lymph vessels. In conclusion, these findings suggest that there exists a marked heterogeneity in the immunohistochemical, genomic, and proliferative activity of human lymphatic endothelial cells between initial and collecting lymph vessels. (*English Translation of J Jpn Coll Angiol, 2008, 48: 125-130.)
収録刊行物
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- Annals of Vascular Diseases
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Annals of Vascular Diseases 5 (3), 269-274, 2012
Annals of Vascular Diseases 編集委員会
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詳細情報
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- CRID
- 1390282680250874112
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- NII論文ID
- 130004487974
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- COI
- 1:STN:280:DC%2BC3srit1Khsw%3D%3D
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- ISSN
- 18816428
- 1881641X
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- PubMed
- 23555524
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可