リニアマイクロスライサを用いた生体内部観察システムの開発  [in Japanese] Development of an Internal Observation System for Biological Samples Using a Linear Micro-slicer  [in Japanese]

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Author(s)

    • 工藤 謙一 KUDOH Ken-ichi
    • 東京大学大学院 工学系研究科 精密機械工学専攻 Department of Precision Engineering, Faculty of Engineering, Tokyo University
    • 木下 泰希 KINOSHITA Taiki
    • 東京大学大学院 工学系研究科 精密機械工学専攻 Department of Precision Engineering, Faculty of Engineering, Tokyo University
    • 都 甲洙 DO Gab-Soo
    • 日本大学生物資源科学部生物環境工学科 Department of Bioenvironmental and Agricultural Engineering, College of Bioresource Sciences, Nihon University
    • 内ヶ崎 万蔵 UCHIGASAKI Manzo
    • 日本大学生物資源科学部生物環境工学科 Department of Bioenvironmental and Agricultural Engineering, College of Bioresource Sciences, Nihon University
    • 佐藤 嘉兵 SATO Kahei
    • 日本大学生物資源科学部応用生物科学科 Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University
    • 樋口 俊郎 HIGUCHI Toshiro
    • 東京大学大学院 工学系研究科 精密機械工学専攻 Department of Precision Engineering, Faculty of Engineering, Tokyo University

Abstract

Recently, the construction of 3-dimensional internal structure images of biological samples has been used for vital observation. To construct the image, the sample is sliced into many sections with a machine such as a microtome. Then images of the sections are acquired by the system. By acquisitioning the images, a 3-dimensional internal structure image of the sample can be created. To realize the mentioned observation system, we proposed a new type of micro-slicer image processing system with a direct translation blade and automatic sample feeding mechanism. An image scanner (contact image sensor) was attached in the system to obtain 2-dimensional images from the sliced sample, not from the sliced section. By using an image scanner called a micro-slicer image processing system instead of a CCD camera, which is used in conventional systems, line lighting of the image scanner enables uniform illumination on the cutting planes of the samples. Additionally, the characteristics of an image scanner, such as shallow depth of field, an oblique illuminating system, and one-dimensional array of light-sensitive elements, enable us to display hollow portions of the sample. As a result, the calibration process of the 2-dimensional images obtained from the sample, which was needed in the conventional system, is no longer necessary. So we achieved the automation of constructing high-definition 3-dimensional images. Furthermore, the miniaturization of equipment was realized because the CCD camera and optical systems became unnecessary.

Journal

  • Transactions of Japanese Society for Medical and Biological Engineering

    Transactions of Japanese Society for Medical and Biological Engineering 43(1), 103-108, 2005

    Japanese Society for Medical and Biological Engineering

Codes

  • NII Article ID (NAID)
    130004493896
  • Text Lang
    JPN
  • ISSN
    1347-443X
  • Data Source
    J-STAGE 
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