Surveillance of Type 1 Ostreid Herpesvirus (OsHV-1) Variants in Japan
Access this Article
Ostreid herpesvirus 1 (OsHV-1) μVar is a variant of OsHV-1 and suspected of being the causative agent of acute mass mortality events of Pacific oysters during summers in Europe since 2008. In this study, the distribution of OsHV-1 was surveyed in the six main oyster-producing areas of Japan, using PCR targeting a C2/C6 fragment including ORF4. PCR products were amplified from 123 out of 1,714 oysters of three species of <i>Crassostrea</i> (<i>C. gigas, C. sikamea</i> and <i>C. ariakensis</i>), and 23 different nucleotide sequences, showing 96% to 99% similarity to the reference OsHV-1, were obtained. Although 18 sequences among the 23 obtained possessed a microsatellite deletion unique to OsHV-1 μVar, all PCR products contained two conserved nucleotides that were shared with the reference OsHV-1 and not with OsHV-1 μVar. Here, we found variable types of OsHV-1 in oysters in Japan, but their nucleotide sequences were not identical to those of OsHV-1 μVar.
- Fish Pathology
Fish Pathology 47(4), 129-136, 2012
The Japanese Society of Fish Pathology