Surveillance of Type 1 Ostreid Herpesvirus (OsHV-1) Variants in Japan

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Author(s)

    • Kurita Jun
    • National Research Institute of Aquaculture, Fisheries Research Agency
    • Kiryu Ikunari
    • National Research Institute of Aquaculture, Fisheries Research Agency
    • Yuasa Kei
    • National Research Institute of Aquaculture, Fisheries Research Agency
    • Kawana Morihiko
    • Hokkaido National Fisheries Research Institute, Fisheries Research Agency
    • Oseko Norihisa
    • National Research Institute of Aquaculture, Fisheries Research Agency

Abstract

Ostreid herpesvirus 1 (OsHV-1) μVar is a variant of OsHV-1 and suspected of being the causative agent of acute mass mortality events of Pacific oysters during summers in Europe since 2008. In this study, the distribution of OsHV-1 was surveyed in the six main oyster-producing areas of Japan, using PCR targeting a C2/C6 fragment including ORF4. PCR products were amplified from 123 out of 1,714 oysters of three species of <i>Crassostrea</i> (<i>C. gigas, C. sikamea</i> and <i>C. ariakensis</i>), and 23 different nucleotide sequences, showing 96% to 99% similarity to the reference OsHV-1, were obtained. Although 18 sequences among the 23 obtained possessed a microsatellite deletion unique to OsHV-1 μVar, all PCR products contained two conserved nucleotides that were shared with the reference OsHV-1 and not with OsHV-1 μVar. Here, we found variable types of OsHV-1 in oysters in Japan, but their nucleotide sequences were not identical to those of OsHV-1 μVar.

Journal

  • Fish Pathology

    Fish Pathology 47(4), 129-136, 2012

    The Japanese Society of Fish Pathology

Codes

  • NII Article ID (NAID)
    130004510244
  • Text Lang
    ENG
  • ISSN
    0388-788X
  • Data Source
    J-STAGE 
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