カリブ海在来 <i>Capsicum chinense</i> 品種の非辛味性,香りおよび来歴の解析 Analysis of Non-pungency, Aroma, and Origin of a <i>Capsicum chinense</i> Cultivar from a Caribbean Island

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Abstract

カリブ海在来のトウガラシ'No.80'は果実の持つ非辛味性および強い芳香性の観点から,果菜類としての非辛味芳香性トウガラシ品種の育種において有望な素材である.本研究では'No.80'の非辛味性,揮発性香り成分および品種の来歴を,同様に非辛味性であるが芳香性の弱いブラジル在来の'No.2'との比較のもと解析した.両品種において <i>acyltransferase</i>(<i>Pun1</i>)の発現およびタンパク質の推定アミノ酸配列は辛味品種'Habanero'と比較して異常が認められなかった.一方,'No.80'および'No.2'の <i>putative aminotransferase</i>(<i>p-AMT</i>)コード領域には,それぞれフレームシフト変異を引き起こす 7 塩基および 8 塩基の挿入が認められた.'Habanero'と'No.80'あるいは'No.2'との交雑後代 F<sub>1</sub> および F<sub>2</sub> における非辛味性と塩基配列の挿入が連鎖したことから,両品種の非辛味性は独立して生じた <i>p-AMT</i> の変異に起因すると考えられた.さらに,両品種の分子系統解析を行ったところ,ブラジル在来の'No.2'と遺伝的に非常に近縁な関係にある'No.80'は,南米大陸に起源を持ち,カリブ海に持ち込まれたことが示唆された.芳香性の強い'No.80',芳香性の弱い'No.2'およびその交雑後代 F<sub>1</sub> の果実における揮発性香り成分を同定・定量した.'No.80'は芳香性に寄与する成分を'No.2'と比較して多量に発散していた.さらに,交雑後代 F<sub>1</sub> は揮発性香り成分の多くを中間あるいは両親以上に発散していた.以上の結果を踏まえて,本研究では多様な非辛味芳香性トウガラシ品種の育種に向けた今後の可能性について考察した.

'No.80' (<i>Capsicum chinense</i>) from the Caribbean is a valuable genetic source from the aspect of its non-pungent and highly aromatic traits. In the present study, the non-pungency, volatile components, and phylogenetic origin of 'No.80' were analyzed with another <i>C. chinense</i> cultivar, 'No.2' from Brazil, which is also non-pungent but less aromatic. Expressions and deduced amino acid sequences of <i>acyltransferase</i> (<i>Pun1</i>) of 'No.80' and 'No.2' were normal compared with a pungent cultivar, 'Habanero'. Insertions of 7-bp and 8-bp resulting in frameshift mutations were found in the coding regions of <i>putative aminotransferase</i> (<i>p-AMT</i>) of 'No.80' and 'No.2', respectively. Co-segregation of these insertions with the non-pungent phenotypes in F<sub>1</sub> and F<sub>2</sub> populations obtained from crossing 'No.80' or 'No.2' with 'Habanero' suggested that non-pungency in these cultivars arose from genetic mutations of <i>p-AMT</i> that occurred independently. Moreover, molecular phylogenetic analysis suggested that 'No.80', a close relative of 'No.2', originates from capsicums migrated from the South American mainland. In addition to pungency, we assessed the volatile components of the highly aromatic 'No.80', the less aromatic 'No.2', and their F<sub>1</sub> hybrid using gas chromatography. 'No.80' contained higher levels of aroma-contributing volatiles than 'No.2', which correlated with the stronger and weaker aromas of two cultivars. Further, the fruit of F<sub>1</sub> progenies emitted a number of volatile compounds between or higher than their corresponding parents. Based on these results, the approaches for breeding highly aromatic non-pungent cultivars are discussed.

Journal

  • Journal of the Japanese Society for Horticultural Science

    Journal of the Japanese Society for Horticultural Science 83(3), 244-251, 2014

    THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE

Codes

  • NII Article ID (NAID)
    130004510827
  • NII NACSIS-CAT ID (NCID)
    AA12177046
  • Text Lang
    ENG
  • ISSN
    1882-3351
  • NDL Article ID
    025584963
  • NDL Call No.
    Z78-A456
  • Data Source
    NDL  J-STAGE 
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