Growth of Protein Crystal at Interface between Two Liquids Using Slow Cooling Method.

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  • Adachi Hiroaki
    Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
  • Watanabe Takahiro
    Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
  • Yoshimura Masashi
    Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
  • Mori Yusuke
    Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
  • Sasaki Takatomo
    Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

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A new method has been developed to grow high-quality and large protein crystals. By a slow cooling method, tetragonal lysozyme crystals were grown from spontaneous nucleation at the interface of two liquids wherein the crystals had no contact with a growth vessel. The long and narrow shape of the crystals indicated that they had grown at a lower supersaturation, leading to high crystallinity. This method also made it possible to remove the crystals easily without causing any mechanical damage. A large lysozyme crystal 2.5 mm in length was obtained in 25 days from a seed crystal kept at the interface. The combination of growth at the interface between two liquids and controlled slow cooling is effective for the growth of high-quality and large protein crystals.

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