Biological Immunoassay Utilizing Magnetic Marker and High T<sub>c</sub>Superconducting Quantum Interference Device Magnetometer

  • Enpuku Keiji
    Research Institute of Superconductor Science and Systems, Kyushu University
  • Kuroda Daisuke
    Research Institute of Superconductor Science and Systems, Kyushu University
  • Ohba Akihiro
    Research Institute of Superconductor Science and Systems, Kyushu University
  • Yang Tong Qing
    Research Institute of Superconductor Science and Systems, Kyushu University
  • Yoshinaga Kohji
    Department of Applied Chemistry, Kyushu Institute of Technology
  • Nakahara Tae
    Department of Applied Chemistry, Kyushu Institute of Technology
  • Kuma Hiroyuki
    Department of Medical Science, Kyushu University
  • Hamasaki Naotaka
    Department of Medical Science, Kyushu University

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タイトル別名
  • Biological Immunoassay Utilizing Magnetic Marker and High Tc Superconducting Quantum Interference Device Magnetometer

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Detection of the biological binding-reaction between an antigen andits antibody was performed by using a magnetic marker and a high Tc superconducting quantum interference device (SQUID) magnetometer. In this method, the binding reaction is detected by measuring the magnetic field from the marker. A new marker made of Fe3O4 particle with diameter of 25 nm was developed, and the remanent field of the marker was used for the detection of the antigen called Interleukin 8 (IL8). It was shown that the present system can detect 0.1 pg weight of IL8.

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