CONSECTIVE MONITORING OF DISSOLVED OXYGEN CONSUMPTION BY DOX SYSTEM TO EVALUATE BACTERIAL CONTAMINATION IN PLATELET CONCENTRATES

• Otsubo Hiroko

  Blood Transfusion Service, Jikei University Hospital Department of Safety Research on Blood and Biological Products, National Institute of infectious Diseases

• Yamaguchi Kazunari

  Department of Safety Research on Blood and Biological Products, National Institute of infectious Diseases

• Hoshi Yasutaka

  Blood Transfusion Service, Jikei University Hospital

Bibliographic Information

Other Title

• 溶存酸素消費量測定による血小板製剤内細菌検出感度についての検討

Abstract

Background: Transfusion-transmitted bacterial infection is a serious and unresolved problem. We evaluated the efficacy of DOX^TM (Daikin Industries) which is a commercially available system that has developed to detect a contaminated food by measuring oxygen potential to detect bacterial contamination in PC.<br> Methods: Staphylococcus aureus, Serratia marcescens, Staphylococcus epidermidis, Bacillus cereus, Streptococcus pneumoniae, and Propionibacterium acnes were inoculated into PC at a final concentration of approximately 10⁰, 10¹ and 10² CFU/ml. Dissolved oxygen potential of the sample taken from each PC just after inoculation was measured consecutively.<br> Results: The detection rate by DOX^TM system in samples inoculated with S. aureus at a concentration of 10⁰, 10¹ and 10²CFU/ml was 28.6%, 78.6% and 85.7%, respectively. That of S. epidermidis was 23.0%, 84.6% and 92.3%, of S. marcescens was 50.0%, 100% and 100%, and of B. cereus was 57.1%, 100% and 100%, respectively. The aerobic organisms were detected in a mean of 7.0 to 18.2 hours.<br> Conclusion: DOX^TM system detected aerobic bacteria in PC within 20hours if their initial concentration was more than 10¹CFU/ml. We consider that this system can be introduced as a useful method to detect the contaminated PC, and can improve the platelet transufusion safety without increasing the medical costs.<br>

Journal

• Japanese Journal of Transfusion and Cell Therapy

  Japanese Journal of Transfusion and Cell Therapy 54 (3), 372-377, 2008

  The Japan Society of Transfusion Medicine and Cell Therapy

Keywords

• Dissolved oxygen consumption
• Platelet concentrates
• bacterial contamination

Details

• CRID: 1390282680249398528
• NII Article ID: 130004542568
• NII Article ID: 10024376307
• NII Book ID: AA12159645
• DOI: 10.3925/jjtc.54.372
• ISSN: 18830625
• ISSN: 18813011
• Web Site: https://search.jamas.or.jp/link/ui/2008257958
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed