The production of VEGF involving MAP kinase activation by low level laser therapy in human granulosa cells

• Kawano Yasushi

  Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University

• Utsunomiya-Kai Yufuko

  Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University

• Kai Kentaro

  Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University

• Miyakawa Isao

  Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University

• Ohshiro Toshio

  Japan Medical Laser Laboratory

• Narahara Hisashi

  Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University

Abstract

Objective: The function of granulosa cells is regulated by various hormones and growth factors. Our aim is to clarify the regulation of vascular endothelial growth factor (VEGF) production via mitogen-activated protein kinase (MAPK) induced by low level laser therapy (LLLT) in human granulosa cells.<br>Methods: A human granulosa cell line, KGN cells, were cultured and incubated after LLLT (60mW, GaAlAs 830nm). The levels of VEGF in the culture media were determined by an enzyme-linked immunosorbent assay. The activation of MAP kinase in KGN cells was detected by western blot analysis.<br>Results: VEGF production was significantly increased by LLLT in a time-dependent manner. MAP kinase activity was increased by LLLT. In addition it was enhanced by LLLT and follicle-stimulating hormone (FSH) stimulation. <br>Conclusions: The results suggested that VEGF is induced by LLLT through mechanisms involving MAPK. The increase in VEGF may contribute to neovascularization, which in turn would promote various ovulation phenomena as well as follicular growth.

Journal

• LASER THERAPY

  LASER THERAPY 21 (4), 269-274, 2012

  International Phototherapy Association

Keywords

• low level laser therapy
• granulosa cell
• VEGF

Details

• CRID: 1390001204632184064
• NII Article ID: 130004553724
• DOI: 10.5978/islsm.12-or-15
• ISSN: 18847269
• ISSN: 08985901
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed