Controlled Calcification using Polyanionic Macromolecules with an Active Reaction Center Analogue of <i>Carbonic Anhydrase</i>

• ABE Shigeaki

  Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University

• ISHIDA Tomoki

  School of Dental Medicine, Hokkaido University

• YAMATOYA Emi

  School of Dental Medicine, Hokkaido University

• HAYASHI Daisuke

  Department of Material Chemistry, Asahikawa National College of Technology

• AKASAKA Tsukasa

  Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University

• UO Motohiro

  Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University

• WATARI Fumio

  Department of Biomedical Materials and Engineering, Graduate School of Dental Medicine, Hokkaido University

• TAKADA Tomoya

  Department of Material Chemistry, Asahikawa National College of Technology

Abstract

Biomimetic macromolecules containing carboxyl and imidazole groups were synthesized. The complex of a zinc ion, a water molecule, and three imidazoles in a polymer is expected to act as an active center analogue of carbonic anhydrase, a hydration enzyme. The obtained polymer complex was applied to calcium carbonate formation. In this study, we investigated the role of both functional groups on the calcification reaction. The presence of a carboxyl group drastically enhanced the biomineralization, because it recognized and condensed the calcium ion at the first step of the calcification reaction. The obtained zinc-imidazole complex selectively induced the formation of vaterite, which is well-known to be a thermodynamically metastable polymorph of calcium carbonate. Interestingly, the results suggest that the active center analogue (zinc-imidazole complex) also stimulates introduction of the HCO₃^- ion to the calcium ion-recognition site.

Journal

• Nano Biomedicine

  Nano Biomedicine 1 (1), 51-58, 2009

  Nano Biomedical Society

Keywords

• biomineralization
• calcium carbonate
• controlled calcification
• carbonic anhydrase
• biomimetic macromolecule

Details

• CRID: 1390282680321900800
• NII Article ID: 130004555789
• DOI: 10.11344/nano.1.51
• ISSN: 21854734
• ISSN: 18835198
• Web Site: https://search.jamas.or.jp/link/ui/2010027630
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed