ヒョウヒダニ類の数とアレルゲンの量的関係

• HASHIMOTO Tomoyuki

  Environmental Biology Department, Japan Environmental Sanitation Center

• KOIZUMI Tomoko

  Environmental Biology Department, Japan Environmental Sanitation Center

• MINAGAWA Keiko

  Environmental Biology Department, Japan Environmental Sanitation Center

• KAZUMA Tohru

  Environmental Biology Department, Japan Environmental Sanitation Center

• MUTO Atsuhiko

  Environmental Biology Department, Japan Environmental Sanitation Center

• EGUCHI Hidenori

  Research & Development Department, R&D and Technical Division.

• MATSUNAGA Tadahiro

  Research & Development Department, R&D and Technical Division.

書誌事項

タイトル別名

• Relationship between the number of <i>Dermatophagoides</i> mites and the quantity of their allergens
• Relationship between the number of Dermatophagoides mites and the quantity of their allergens

抄録

Quantities of house dust mite allergens, Der 1 and Der 2, extracted from artificially-reared Dermatophagoides farinae Hughes (Df) were compared. Relationships among the allergen levels, numbers of mites (1-1,000 mites), and life stages (adult females and larvae) were analyzed. The mite samples were prepared with or without grinding. The adult females of D. pteronyssinus (Trouessart) (Dp) were also examined after grinding. The quantities of Der 1 and Der 2 were determined by ELISA using monoclonal antibodies. Overall, the allergen quantities increased depending on the numbers of mites. The Der 1 levels of 100 mites calculated were 34.6 (Df larvae) to 304 (Dp females) ng. The Der 2 levels per 100 mites were also equivalent to 85.2 (Df larvae) to 758 (Dp females) ng. The ratio of Der 1 : Der 2 was 1 : 0.95-2.65. The Df females showed significantly higher levels of allergens than the larvae. The Dp females yielded larger amounts of Der 2 than Df females, but the differences in Der 1 were not significant. The commercialized test kits to inspect mite infestation detect Der 2. There is not a standard of Der 2 for allergen avoidance, so determination of Der 2 standard is required.

収録刊行物

• 衛生動物

  衛生動物 61 (4), 345-351, 2010

  日本衛生動物学会

キーワード

• house dust mites
• <i>Dermatophagoides</i>
• allergen
• Der 1
• Der 2
• ELISA

詳細情報

• CRID: 1390282679918144384
• NII論文ID: 130004558981
• NII論文ID: 10027435162
• NII書誌ID: AN00021948
• DOI: 10.7601/mez.61.345
• ISSN: 21855609
• ISSN: 04247086
• NDL書誌ID: 10933305
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10933305
• Web Site: https://search.jamas.or.jp/link/ui/2011110564
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可