イヌにおける<i>UCP2</i>および<i>UCP3</i> cDNAの単離と発現解析  [in Japanese] cDNA cloning and expression analysis of canine <i>uncoupling protein</i> 2 and 3 genes  [in Japanese]

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Author(s)

    • 宇田川 智野 Udagawa Chihiro
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 鄭 英和 Chong Yong Hwa
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 市東 正幸 Shito Masayuki
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 河上 剛 Kawakami Tsuyoshi
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 多田 尚美 Tada Naomi
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 落合 和彦 Ochiai Kazuhiko
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 石岡 克己 Ishioka Katsumi
    • 日本獣医生命科学大学 Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 土田 修一 Tsuchida Shuichi
    • 日本獣医生命科学大学 Laboratory of Comparative Cellular Biology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
    • 近江 俊徳 Omi Toshinori
    • 日本獣医生命科学大学 Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University

Abstract

ミトコンドリア脱共役蛋白質(uncoupling protein;UCP)は、ミトコンドリア内膜での酸化的リン酸化反応を脱共役させ、エネルギーを熱として散逸する機能を有している。<i>UCP2</i>遺伝子と<i>UCP3</i>遺伝子は、<i>UCP</i>ファミリーに属する分子でイヌでは21番染色体にタンデムに位置している。我々は、今回ビーグル犬由来の骨格筋よりイヌUCP2とイヌUCP3の5,非翻訳領域および翻訳領域のcDNA単離を試み、<i>UCP2</i> cDNA 1251bp(GenBank Accession No. AB611704)および<i>UCP3</i> cDNA 1301bp(GenBank Accession No. AB611705)の塩基配列を決定した。また、イヌゲノム構造およびヒトの<i>UCP</i>遺伝子構造との比較解析により、ヒト同様にイヌ<i>UCP2</i>遺伝子は8つのエクソンをまたイヌ<i>UCP3</i>遺伝子は7つのエクソンで構成されていることを明らかとした。27種類の組織由来のtotal RNA(市販品)を用いたRT-PCR法による遺伝子発現解析では、両遺伝子はこれまで報告されている通り、組織での遺伝子発現パターンに差異が認められた。ヒトにおいては、<i>UCP2</i>や<i>UCP3</i>は<i>UCP1</i>と共に、肥満や糖尿病に関連している事が明らかになっている。今回の結果は、イヌにおけるエネルギー代謝のバランスに両遺伝子がどのような影響を及ぼしているのかを分子遺伝学的に解析するために必要な基礎的知見と考える。

The uncoupling proteins (UCPs) play an important role in energy homeostasis. To date, five UCP homologs, UCP1 to UCP5, have been identified in mammals. Both UCP2 and UCP3 have been found to decrease membrane potential and increase thermogenesis. They are regarded as inducers of obesity and type 2 diabetes in humans. We showed that the dog UCP2 and UCP3 cDNAs consisted of a part of the 5'-UTR sequence and a complete open reading frame in each gene. Comparisons with the gene structure in humans, dog genome sequences, and the transcripts in this study showed that dog <i>UCP2</i> consists of exons 1 to 8 and dog <i>UCP3</i> consists of exons 1 to 7. In dog <i>UCP 3</i>, exon 3-skipped transcripts were also observed. A <i>UCP2</i> and <i>UCP3</i> gene expression study using reverse transcription-polymerase chain reaction and 27 kinds of total RNA from dog tissues showed that tissue distribution of gene expression was different between dog <i>UCP2</i> and <i>UCP3</i>. The present data may provide us with information regarding DNA polymorphism and help in further investigations of the influence of UCP2 and UCP3 activity on canine energy balance.

Journal

  • Journal of Pet Animal Nutrition

    Journal of Pet Animal Nutrition 14(2), 68-75, 2011

    Japanese Society of Pet Animal Nutrition

Codes

  • NII Article ID (NAID)
    130004565633
  • Text Lang
    JPN
  • ISSN
    1344-3763
  • Data Source
    J-STAGE 
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