<b>Comparative study of the biological effects of antimicrobials, triclosan and trichlocarban, for </b><i><b>C. elegan</b></i><i><b>s </b></i>

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Author(s)

    • Inokuchi Ayako
    • Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
    • Nihira Moritoshi
    • Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
    • Minakoshi Midori
    • Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
    • Yamamoto Ryoko
    • Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
    • Tominaga Nobuaki
    • Department of Chemical and Biological Engineering, Ariake National College of Technology
    • Arizono Koji
    • Department of Chemical and Biological Engineering, Ariake National College of Technology

Abstract

Triclosan (TCS) and trichlocarban (TCC) are used as antimicrobials in soaps and other household products, because of their antibacterial and anti-fungal properties. We assessed the potential toxicological effects associated with TCS and TCC using <i>Caenorhabditis elegans </i>(<i>C. elegans</i>) as a model soil organism. We examined changes in the test endpoints of growth and maturation such as body length,ratio (%) of gravid worms and fecundity. Growth tests showed that the lowest observed effective concentrations (LOEC) of TCS and TCC in <i>C. elegans </i>were 0.78 and <0.074 mg/L, respectively. The estimated LOEC values in maturation tests were 3.13 mg/L for TCS and 0.17 mg/L for TCC. The estimated LOEC for TCC in reproduction tests was 0.11 mg/L, but this could not be determined for TCS (>6.25 mg/L). We also investigated the suitability of the DNA microarray as an eco-toxicogenomic approach to determine the physiological function of TCS and TCC in <i>C. elegans</i>. Both TCS (5.0 mg/L) and TCC (0.166 mg/L) were examined mainly as cytochrome P450 (CYP) gene expression using a customized chip for <i>C. elegans</i>. Among 79 CYP genes, 19 and 10 were up-regulated upon exposure to TCS and TCC, respectively. Both TCS (5.0 mg/L) and TCC (0.166 mg/L) were common to indicate the induction ability of CYP35As, which function is related to mammalian CYP2 clade.

Journal

  • Journal of Environment and Safety

    Journal of Environment and Safety 5(2), 95-98, 2014

    Academic Consociation of Environmental Safety and Waste Management, Japan

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