トランスクリプトームとメタボロームの融合
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- 平井 優美
- 理研植物科学研究センター
書誌事項
- タイトル別名
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- Integration of transcriptomics and metabolomics
抄録
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life. The integrated transcriptome and metabolome analyses lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. Here we report the discovery of two transcription factors that positively control the biosynthesis of glucosinolates, a group of bioactive secondary products, in Arabidopsis thaliana by an integrated omics approach. Combined transcriptome coexpression analysis of publicly-available condition-independent data, and the condition-specific data identified Myb28 and Myb29 as candidate transcription factor genes specifically involved in the regulation of aliphatic glucosinolate production. Analysis of a knockout mutant demonstrated that Myb28 is a positive regulator for basal-level production of aliphatic glucosinolates. Myb29 presumably plays an accessory function for methyl jasmonate-mediated induction of a set of aliphatic glucosinolate biosynthetic genes. Overexpression of Myb28 in Arabidopsis cultured suspension cells, which don't normally synthesize glucosinolates, resulted in the production of large amounts glucosinolates, suggesting the possibility of efficient industrial production of glucosinolates by manipulation of these transcription factors. A working model for regulation of glucosinolate production involving these genes, renamed Production of Methionine-derived Glucosinolate (PMG) 1 and 2, are postulated.
収録刊行物
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- ケモインフォマティクス討論会予稿集
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ケモインフォマティクス討論会予稿集 2007 (0), JL01-JL01, 2007
公益社団法人 日本化学会・情報化学部会
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キーワード
詳細情報
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- CRID
- 1390001205736124160
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- NII論文ID
- 130004574963
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可