PCR法による唾液中の単純ヘルペスウイルス検出に関する研究  [in Japanese] Detection of Herpes Simplex Virus Existence in the Saliva of Pediatric Patients using the Polymerase Chain Reaction  [in Japanese]

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Abstract

口腔内に潜伏する単純ヘルペスウイルスの検出を目的として,本学歯学部附属病院小児歯科外来を受診した,口腔内に疱疹性歯肉口内炎および口唇ヘルペスを発症していない健常児241名(年齢0歳2か月~14歳8か月)の唾液を採取して検体とし,PCR法を用いてHSVの検出を行い,以下の結果を得た。<BR>1)検体試料採取時,口腔内に疱疹性歯肉口内炎および口唇ヘルペスを発症していない健常児を対象としたにもかかわらず,被験児唾液中から17.0%の割合でHSVが検出された。<BR>2)年齢別のHSV検出率では,0歳および1歳において0%であり,2歳以降においてほぼ一定の検出率であった。<BR>3)兄弟姉妹の間の潜伏状況を調べると,兄弟姉妹全員がHSV(+)である群と兄弟姉妹のうち少なくとも1人がHSV(+)である群との間に有意差はなかった。

The aim of the present study was to evaluate the existence of the Herpes Simplex Virus (HSV) in the saliva. HSV rarely evokes herpetic gingivostomatitis and herpetic labialis with an intensive mucosal manifestation in healthy pediatric patients. To detect the HSV, rapid and sensitive Polymerase Chain Reaction (PCR) was employed. At pediatric outpatient clinic of Hokkaido University Dental Hospital, asorbent cotton swabs were randomly dipped into the saliva of 241 healthy children (aged from 0 to 14) who had no symptoms of gingival or mucosal inflammation.<BR>The rapid and sensitive PCR method revealed that HSV was distributed among healthy children in the pediatric outpatient clinic, showing the etiological significance.<BR>The results were as follows:<BR>1) HSV positive findings were revealed in 17.0% of the total samples from healthy children with no manifestation.<BR>2) HSV positive findings were revealed at 0% in both 1 and 2 year old children and they had significantly lower rates than those of the other ages.<BR>3) Investigating the evidence of HSV in siblings revealed that the HSV-positive rate detected in all siblings in the same family was not significantly different from the rate found in one of siblings in the same family.

Journal

  • The Japanese Journal of Pediatric Dentistry

    The Japanese Journal of Pediatric Dentistry 35(5), 773-777, 1997

    The Japanese Society of Pediatric Dentistry

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