プロスタグランジンF<sub>2α</sub>及び性腺刺激ホルモンによる排卵同期化法を用いた豚の定時人工授精  [in Japanese] Fixed-Time Artificial Insemination Protocol after Treatment for Synchronization of Ovulation with a Combination of Prostaglandin F<sub>2α </sub>and Gonadotropins in Pigs  [in Japanese]

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Abstract

プロスタグランジンF<sub>2α</sub>(PGF<sub>2α</sub>),馬絨毛性性腺刺激ホルモン及びヒト絨毛性性腺刺激ホルモン(hCG)投与による排卵同期化法を用いた豚の定時人工授精(AI)技術の確立を目的とし,排卵同期化処置を施した豚の生殖内分泌動態,排卵時期及びAI適期を検討した.排卵後12日から排卵同期化処置を施した豚の血中プロジェステロン濃度は,PGF<sub>2α</sub>投与の翌日には有意に低下した.排卵前に起こる黄体形成ホルモンサージのピークは,hCG投与後26.0±1.2時間に認められ,排卵はhCG投与後44.8±2.3時間に観察された.発情終了後から処置を開始し,hCG投与後24時間あるいは36時間に1回のみAIを行った場合,すべての豚から正常な形態を示す胚盤胞が回収された.また,hCG投与後24時間あるいは36時間に1回のみAIを行った豚の繁殖成績は,自然発情中に複数回AIを行った豚と同等であることが明らかとなった.

To establish a fixed-time artificial insemination (AI) protocol in pigs after treatment for synchronization of ovulation with a combination of prostaglandin F<sub>2α</sub> (PGF<sub>2α</sub>), equine chorionic gonadotropin and human chorionic gonadotropin (hCG), we investigated the reproductive hormone profiles and ovulation in the synchronized sows and determined a satisfactory timing for a single AI to provide sufficient reproductive performance. Plasma progesterone concentrations significantly decreased on the day following PGF<sub>2α</sub> treatment. A preovulatory surge of luteinizing hormone was observed in all synchronized sows, peaking at 26.0±1.2 hours after hCG treatment, and ovulation was detected at 44.8±2.3 hours after treatment. When a timed AI was performed once at 24 or 36 hours after hCG treatment, normal blastcysts were collected from all of the synchronized pigs. Moreover, farrowing rate and average litter size did not differ between the synchronized pigs inseminated once at 24 or 36 hours after hCG treatment and cyclic pigs inseminated multiple times during natural estrus.

Journal

  • Journal of the Japan Veterinary Medical Association

    Journal of the Japan Veterinary Medical Association 67(2), 119-124, 2014

    Japan Veterinary Medical Association

Codes

  • NII Article ID (NAID)
    130004640744
  • NII NACSIS-CAT ID (NCID)
    AN00191857
  • Text Lang
    JPN
  • Article Type
    journal article
  • ISSN
    0446-6454
  • NDL Article ID
    025252361
  • NDL Call No.
    Z18-352
  • Data Source
    NDL  J-STAGE  JASI 
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