Detection of Anti Porcine Reproductive and Respiratory Syndrome Virus Antibody and Specific Virus Gene from Oral Fluids

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  • 口腔液を用いた豚繁殖・呼吸障害症候群ウイルス抗体及び遺伝子の検出
  • コウコウエキ オ モチイタ ブタ ハンショク ・ コキュウ ショウガイ ショウコウグン ウイルス コウタイ オヨビ イデンシ ノ ケンシュツ

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Abstract

From 14 swine herds in Niigata prefecture, serum and pen-based oral fluid samples were collected from 50 pig groups aged two to eight months. The specimens were assayed for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection using the indirect fluorescent antibody test (IFAT), ELISA and RT-nested PCR. With both IFAT and ELISA, 40 groups were positive and 10 were negative in the serum samples, and 38 groups were positive and 12 were negative in the oral fluids (96% matched). In terms of the detection of the PRRSV specific gene using RT-nested PCR, 18 groups were positive and 32 groups were negative in the serum samples, and 15 groups were positive and 35 groups were negative in the oral fluids (90% matched). Oral fluid sampling may contribute to the development of high efficiency, low cost PRRSV surveillance in pig groups.

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