実験動物サルのMHCクラスI多様性  [in Japanese] Diversity of MHC class I genes in rhesus macaques and cynomolgus macaques (crab-eating monkeys)  [in Japanese]

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Author(s)

    • 成瀬 妙子 Naruse Taeko
    • 東京医科歯科大学難治疾患研究所分子病態分野 Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University
    • 木村 彰方 Kimura Akinori
    • 東京医科歯科大学難治疾患研究所分子病態分野 Department of Molecular Pathogenesis, Medical Research Institute, Tokyo Medical and Dental University

Abstract

旧世界ザルに属するアカゲザル(<i>Macaca mulatta</i>)やカニクイザル(<i>Macaca fascicularis</i>)は,ヒトに最も近い重要な実験動物として,エイズワクチン開発や薬剤の安全性確認をはじめとする各種の医学研究に欠かせない役割を担っている。このような動物実験,なかでも免疫に関わる実験の結果を正しく評価するためには,各個体の免疫応答に関わる遺伝的背景,特にMHC情報を把握することが重要である。筆者らの研究室では,以前よりエイズワクチン開発モデルであるサル免疫不全症候群ウイルス(simian immunodeficiency virus: SIV)に対するワクチン開発実験に用いられているアカゲザルやカニクイザルについて,RSCA(reference strand-mediated conformation analysis)法やcDNAクローニングにより<i>Mhc</i>クラスI遺伝子群の解析を行い,多数の新規アリルを同定する一方,家系解析などから旧世界ザルに特徴的なMHC ハプロタイプ構造を見出している。本稿では,旧世界ザル<i>Mhc</i>クラスI解析に関する研究の現状を紹介する。

Non-human primates are widely used in medical researches including development of vaccines against pathogens, because their immune system is similar to that in humans. In particular, the Old World monkeys such as rhesus macaques (<i>Macaca mulatta</i>) and cynomolgus macaques (crab-eating macaques, <i>Macaca fascicularis</i>) are useful models for human infectious diseases, especially for developing a strategy for vaccination against HIV, by using simian immunodeficiency virus (SIV) infection as a model system. Because there are individual differences in immune responsiveness, which are controlled by the polymorphic nature of the major histocompatibility (MHC) locus, it is important to reveal the diversity of MHC in the model animal. We analyzed polymorphisms in <i>Mhc</i> class I loci in rhesus macaques (<i>Mamu</i> locus) from Myanmar (Burma) and Laos and cynomolgus macaques (<i>Mafa</i> locus) from Indonesia, Malaysia and Philippine to obtain genetic information about the role of <i>Mhc</i> class I diversity in the immune responsiveness. We found that each <i>Mhc</i> class I haplotype was composed of one to three <i>Mamu-A</i> or <i>Mafa-A</i> alleles and one to five <i>Mamu-B</i> or <i>Mafa-B</i> alleles. In addition, family studies revealed that there were three <i>Mhc</i> haplotypes carrying two <i>Mhc-A1</i> alleles not only in rhesus but also in cynomolgus macaques. These observations further demonstrated the complexity of <i>Mhc</i> class I loci in the Old World monkeys.

Journal

  • Major Histocompatibility Complex

    Major Histocompatibility Complex 20(1), 35-44, 2013

    Japanese Society for Histocompatibility and Immunogenetics

Codes

  • NII Article ID (NAID)
    130004647537
  • Text Lang
    JPN
  • ISSN
    2186-9995
  • Data Source
    J-STAGE 
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