Nanostructural Control of Biological Molecules Arranged by Using Langmuir-Blodgett Films of Organo-modified Alminosilicate as a Template

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Abstract

Ultrathin films of regularly adsorbed biological molecules have been fabricated by means of the modified Langmuir-Blodgett (LB) method using an organo-modified alminosilicate (montmorillonite). In this combined methods, clay LB films play a template role in the formation of the lysozyme thin layer. Monolayer behavior on the buffer solution containing lysozyme molecules monitored by pressure-area isotherms. Confirmation of chemisorption of biological molecules to the anionic montmorillonite surface is performed by comparison between IR spectra of multilayers of organo-clay and one chemisorbed lysozyme. The bands of N-H and C=O stretching vibration of lysozyme were clearly confirmed on the spectra of multilayers chemisorbed biomolecules. Surface morphology of these monolayers was observed by atomic force microscopy. The difference between height information of monolayers for only organo-modified alminosilicates and their adsorbed lysozyme indicates about 9 nm. This value is almost corresponded to twice for length of lysozyme along the long axis. Hence, formation of double layered structure of biomolecule on the montmorillonite surface was assumed in this system.

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