RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method

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Author(s)

    • Yamada Yuma
    • Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University
    • Harashima Hideyoshi
    • Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University|Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University

Abstract

The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 37(8), 1411-1415, 2014

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130004677537
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    025610303
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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