Observation of Glycolytic Metabolites in Tumor Cell Lysate by Using Hyperpolarization of Deuterated Glucose

• Kumagai Keiko

  Science Research Center, Kochi University

• Akakabe Mai

  Science Research Center, Kochi University

• Tsuda Masayuki

  Science Research Center, Kochi University

• Tsuda Masashi

  Center for Advanced Marine Core Research, Kochi University

• Fukushi Eri

  Research Faculty of Agriculture, Hokkaido University

• Kawabata Jun

  Research Faculty of Agriculture, Hokkaido University

• Abe Takamasa

  Oxford Instruments KK

• Ichikawa Kazuhiro

  Innovation Center for Medical Redox Navigation, Kyushu University

Abstract

Hyperpolarization of stable isotope-labeled substrates and subsequent NMR measurement of the metabolic reactions allow for direct tracking of cellular reactions in vitro and in vivo. Here, we report the hyperpolarization of ¹³C₆-glucose-d₇ and evaluate its use as probes to observe glucose flux in cells. We measured the lifetime of the polarized signal governed by the spin–lattice relaxation time T₁. ¹³C₆-Glucose-d₇ exhibited a T₁ that was over ten times as long as that of ¹³C₆-glucose, and metabolic NMR studies of hyperpolarized ¹³C₆-glucose-d₇ using tumor cell lysate led to observation of the resonances due to phosphorylated fluctofuranoses generated through aerobic glycolysis.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 37 (8), 1416-1421, 2014

  The Pharmaceutical Society of Japan

Keywords

• dynamic nuclear polarization
• glucose
• metabolic reaction
• spin–lattice relaxation time (<i>T</i>₁)

Details

• CRID: 1390001204632210816
• NII Article ID: 130004677538
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b14-00156
• COI: 1:STN:280:DC%2BC2cbos1Khtg%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 025610325
• PubMed: 25087964
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025610325
• Web Site: https://www.jstage.jst.go.jp/article/bpb/37/8/37_b14-00156/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015097901
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed