Interaction-dependent Native Chemical Ligation and Enzyme Reconstitution for Detection of Peptide–Peptide Interaction

• Tsuyoshi Takahashi

  Advanced Scientific Research Leaders Development (ASRLD) Unit, Gunma University

• Akinori Saito

  Division of Molecular Science, Faculty of Science and Technology, Gunma University

Abstract

A new detection method for ligand–protein interactions was constructed using interaction-dependent native chemical ligation and enzyme reconstitution (IDNCL-ER) by employing ribonuclease S. Using a coiled-coil peptide pair as a model of ligand–protein interactions, we determined that IDNCL-ER can work well to detect the peptide–peptide interactions by enzymatic activity.

Journal

• Chemistry Letters

  Chemistry Letters 43 (8), 1357-1359, 2014-05-14

  Oxford University Press (OUP)

Keywords

• General Chemistry

Details

• CRID: 1360565169058977664
• NII Article ID: 130004677586
• DOI: 10.1246/cl.140437
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/43/8/1357/55640573/cl.140437.pdf
• Data Source:
  • Crossref
  • CiNii Articles