Effect of Immobilization on Insoluble Collagen Concentration and Type I and Type III Collagen Isoforms of Rat Soleus Muscle

• Hibino Itaru

  Department of Rehabilitation Science, Faculty of Human Health, Nagoya Gakuin University Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University

• Okita Minoru

  Division of Physical Therapy and Occupational Therapy Science, Graduate School of Health Sciences, Nagasaki University

• Inoue Takayuki

  Program in Physical and Occupational Therapy, Graduate School of Health Sciences, Nagoya University

• Banno Yasuhiro

  Faculty of Care and Rehabilitation, Seijoh University

• Hoso Masahiro

  Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University

Abstract

Immobilization is often associated with decreased muscle elasticity. This condition is known as muscle contracture; however, the mechanism remains unclear. The purpose of this study was to clarify the mechanism governing muscle contracture in rat soleus muscle by identifying changes in ankle joint mobility, insoluble collagen concentration and type I and type III collagen isoforms following 1- and 3-week immobilizations. Following a 1-week immobilization, range of motion (ROM) of dorsiflexion declined to 90% of the control value; additionally, ROM dropped to 67.5% of the control value after a 3-week immobilization. This finding suggested that ankle joint mobility decreases in conjunction with extended periods of immobilization. Insoluble collagen concentration in soleus muscles, which was unchanged after 1 week of immobilization, increased 3 weeks after immobilization. These results may be indicative of collagen fibers with strong intermolecular cross-links contained in the muscle was made increased relatively by 3 weeks of immobilization. Therefore, the change in intermolecular cross-links may be significant in terms of progress of muscle contracture with longer periods of immobilization. On the other hand, the ratio of type III to type I collagen isoforms in muscular tissue increased following a 1-week immobilization; moreover, this ratio remained constant after 3 weeks of immobilization. These data suggested that muscle immobilization may induce type III collagen isoform expression. The increase in the ratio of type III to type I collagen isoforms do not change in parallel with the increase in the limitation in ROM; however, this phenomenon probably is not closely related to the progress of muscle contracture. The change of collagen isoform in immobilized muscle may be involved in the mechanism governing the progression of muscle fibrosis.<br>

Journal

• Journal of the Japanese Physical Therapy Association

  Journal of the Japanese Physical Therapy Association 11 (1), 1-6, 2008

  JAPANESE PHYSICAL THERAPY ASSOCIATION

Keywords

• immobilization
• muscle contracture
• collagen

Details

• CRID: 1390282679446191488
• NII Article ID: 130004677668
• NII Book ID: AA12022775
• DOI: 10.1298/jjpta.11.1
• ISSN: 21888361
• ISSN: 13441272
• HANDLE: 10069/16900
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/20931
• Web Site: http://www.jstage.jst.go.jp/article/jjpta/11/1/11_1_1/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008324174
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed