Identification and Characterization ofVancomycin-resistant <i>Enterococcus</i> species Frequently Isolated from Laboratory Mice

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Author(s)

    • YAMANAKA Hitoki Yamanaka Hitoki
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
    • Yamamoto Naoto
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
    • Kubo Noriaki
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
    • Takemoto Takahira
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
    • Sasano Shoko
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
    • Masuyama Ritsuko
    • Department of Molecular Bone Biology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan
    • Ohsawa Kazutaka
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan

Abstract

To determine the prevalence of drug resistant bacteria colonizing laboratory mice, we isolated and characterized vancomycin-resistant <i>Enterococcus</i> species (VRE) from commercially available mice. A total of 24 VRE isolates were obtained from 19 of 21 mouse strains supplied by 4 commercial breeding companies. Of these, 19 isolates of <i>E. gallinarum</i> and 5 isolates of <i>E. casseliflavus</i> possessing the <i>vanC1</i> and <i>vanC2/3</i> genes intrinsically, exhibited intermediate resistance to vancomycin respectively. In addition, these isolates also exhibited diverse resistant patterns to erythromycin, tetracycline, and ciprofloxacin, whereas the use of antibiotics had not been undertaken in mouse strains tested in this study. Although 6 virulence-associated genes (<i>ace</i>, <i>asa</i>, <i>cylA</i>, <i>efaA</i>, <i>esp</i>, and <i>gelE</i>) and secretion of gelatinase and hemolysin were not detected in all isolates, 23 of 24 isolates including the isolates of <i>E. casselifalvus</i> secreted ATP into culture supernatants. Since secretion of ATP by bacteria resident in the intestinal tract modulates the local immune responses, the prevalence of ATP-secreting VRE in mice therefore needs to be considered in animal experiments that alter the gut microflora by use of antibiotics.

Journal

  • Experimental Animals

    Experimental Animals 63(3), 297-304, 2014

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130004677820
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1341-1357
  • NDL Article ID
    025599157
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  IR  J-STAGE 
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