<i>In Vivo</i> image Analysis Using iRFP Transgenic Mice

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Author(s)

    • TRAN Mai Thi Nhu Tran Mai Thi Nhu
    • Department of Anatomy and Embryology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • TANAKA Junko Tanaka Junko
    • Department of Molecular Pharmacology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • HAMADA Michito [他] Hamada Michito
    • Department of Anatomy and Embryology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Sugiyama Yuka
    • Department of Molecular Pharmacology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Sakaguchi Shota
    • Department of Molecular Pharmacology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Nakamura Megumi
    • Department of Anatomy and Embryology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Takahashi Satoru
    • Department of Anatomy and Embryology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan
    • Miwa Yoshihiro
    • Department of Molecular Pharmacology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan

Abstract

Fluorescent proteins with light wavelengths within the optical window are one of the improvements in <i>in vivo</i> imaging techniques. Near-infrared (NIR) fluorescent protein (iRFP) is a stable, nontoxic protein that emits fluorescence within the NIR optical window without the addition of exogenous substrate. However, studies utilizing an <i>in vivo</i> iRFP model have not yet been published. Here, we report the generation of transgenic iRFP mice with ubiquitous NIR fluorescence expression. iRFP expression was observed in approximately 50% of the offspring from a matings between iRFP transgenic and WT mice. The serum and blood cell indices and body weights of iRFP mice were similar to those of WT mice. Red fluorescence with an excitation wavelength of 690 nm and an emission wavelength of 713 nm was detected in both newborn and adult iRFP mice. We also detected fluorescence emission in whole organs of the iRFP mice, including the brain, heart, liver, kidney, spleen, lung, pancreas, bone, testis, thymus, and adipose tissue. Therefore, iRFP transgenic mice may therefore be a useful tool for various types of <i>in vivo</i> imaging.

Journal

  • Experimental Animals

    Experimental Animals 63(3), 311-319, 2014

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130004677824
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • ISSN
    1341-1357
  • NDL Article ID
    025599184
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  J-STAGE 
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