Identification of <i>Stmm3</i> locus Conferring Resistance to Late-stage Chemically Induced Skin Papillomas on Mouse Chromosome 4 by Congenic Mappingand Allele-specific Alteration Analysis

• Saito Megumi

  Department of Carcinogenesis Research, Division of Experimental Animal Research, Chiba Cancer Center Research Institute, 666-2 Nitonacho, Chuouku, Chiba 260-8717, Japan

• Okumura Kazuhiro

  Department of Carcinogenesis Research, Division of Experimental Animal Research, Chiba Cancer Center Research Institute, 666-2 Nitonacho, Chuouku, Chiba 260-8717, Japan

• Miura Ikuo

  Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, Riken Bioresource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

• Wakana Shigeharu

  Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, Riken Bioresource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

• Kominami Ryo

  Department of Molecular Genetics, Graduate School of Medical and Dental Sciences, Niigata University, Asahimachi 1-757, Niigata 951-8510, Japan

• Wakabayashi Yuichi

  Department of Carcinogenesis Research, Division of Experimental Animal Research, Chiba Cancer Center Research Institute, 666-2 Nitonacho, Chuouku, Chiba 260-8717, Japan

Bibliographic Information

Other Title

• Identification of Stmm3 locus Conferring Resistance to Late-stage Chemically Induced Skin Papillomas on Mouse Chromosome 4 by Congenic Mapping and Allele-specific Alteration Analysis

Abstract

Genome-wide association studies have revealed that many low-penetrance cancer susceptibility loci are located throughout the genome; however, a very limited number of genes have been identified so far. Using a forward genetics approach to map such loci in a mouse skin cancer model, we previously identified strong genetic loci conferring resistance to chemically induced skin papillomas on chromosome 4 and 7 with a large number of [(FVB/N × MSM/Ms) F₁ × FVB/N] backcross mice. In this report, we describe a combination of congenic mapping and allele-specific alteration analysis of the loci on chromosome 4. We used linkage analysis and a congenic mouse strain, FVB.MSM-Stmm3 to refine the location of Stmm3 (Skin tumor modifier of MSM 3) locus within a physical interval of about 34 Mb on distal chromosome 4. In addition, we used patterns of allele-specific imbalances in tumors from N₂ and N₁₀ congenic mice to narrow down further the region of Stmm3 locus to a physical distance of about 25 Mb. Furthermore, immunohistochemical analysis showed papillomas from congenic mice had less proliferative activity. These results suggest that Stmm3 responsible genes may have an influence on papilloma formation in the two-stage skin carcinogenesis by regulating papilloma growth rather than development.

Journal

• Experimental Animals

  Experimental Animals 63 (3), 339-348, 2014

  Japanese Association for Laboratory Animal Science

Keywords

• allelic imbalance
• congenic mice
• DMBA-TPA
• papilloma
• tumor susceptibility

Details

• CRID: 1390001205045233664
• NII Article ID: 130004677828
• NII Book ID: AA11032321
• DOI: 10.1538/expanim.63.339
• COI: 1:STN:280:DC%2BC2cbnvVGqsg%3D%3D
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL BIB ID: 025599270
• PubMed: 25077764
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025599270
• Web Site: https://www.jstage.jst.go.jp/article/expanim/63/3/63_14-0021/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015113914
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed