Reconstitution of intracellular environments <italic>in vitro</italic> and in artificial cells

• Fujiwara Kei

  Department of Biosciences & Informatics, Keio University

• Yanagisawa Miho

  Department of Applied Physics, Tokyo University of Agriculture and Technology

• Nomura Shin-ichiro M.

  Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University

書誌事項

タイトル別名

• Reconstitution of intracellular environments <i>in vitro</i> and in artificial cells
• Reconstitution of intracellular environments in vitro and in artificial cells

抄録

Toward reconstitution of living cells by artificial cells technology, it is critical process to understand the differences between mixtures of biomolecules and living cells. For the aim, we have developed procedures for preparation of an additive-free cell extract (AFCE) and for concentrating biomacromolecules in artificial cells. In this review, we introduce our recent progress to reconstitute intracellular environments in vitro and in artificial cells.

収録刊行物

• BIOPHYSICS

  BIOPHYSICS 10 (0), 43-48, 2014

  日本生物物理学会

キーワード

• Liposome
• cell-free protein expression
• molecular crowding
• cell reconstitution

詳細情報

• CRID: 1390001205222828672
• NII論文ID: 130004678426
• DOI: 10.2142/biophysics.10.43
• ISSN: 13492942
• Web Site: https://www.jstage.jst.go.jp/article/biophysics/10/0/10_43/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可