Structural specificity of electric potentials in the coulometric-array analysis of catechins and theaflavins

Access this Article

Author(s)

    • Matsuura Kanako
    • Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
    • Usui Yumiko
    • Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
    • Kan Toshiyuki
    • Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
    • Ishii Takeshi
    • Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
    • Nakayama Tsutomu
    • Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka|Department of Food Science and Technology, Nippon Veterinary and Life Science University

Abstract

We have established a novel method to evaluate the redox properties of tea polyphenols by HPLC-coulometric-array analysis. We plotted the quantity of electricity (µC) on the vertical axis and the electric potential (mV), adjusted with the associated palladium reference electrode, on the horizontal axis to provide "quantity versus potential (QP) plot". The patterns of the plots correspond to the derivative of a hydrodynamic voltammogram or a current-voltage curve, with the electric potentials of the peaks in the QP plot corresponding to the half-wave potentials in the current-voltage curve. We confirmed that catechins and theaflavins are oxidized depending on the electric potentials of their partial structures, and found that all compounds showing a peak at 0 mV in the QP plots produce hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) during the autoxidation process.

Journal

  • Journal of Clinical Biochemistry and Nutrition

    Journal of Clinical Biochemistry and Nutrition 55(2), 103-109, 2014

    SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Codes

  • NII Article ID (NAID)
    130004678427
  • Text Lang
    ENG
  • ISSN
    0912-0009
  • Data Source
    J-STAGE 
Page Top