Structural specificity of electric potentials in the coulometric-array analysis of catechins and theaflavins
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- Matsuura Kanako
- Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
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- Usui Yumiko
- Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
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- Kan Toshiyuki
- Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
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- Ishii Takeshi
- Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka
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- Nakayama Tsutomu
- Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka Department of Food Science and Technology, Nippon Veterinary and Life Science University
抄録
We have established a novel method to evaluate the redox properties of tea polyphenols by HPLC-coulometric-array analysis. We plotted the quantity of electricity (µC) on the vertical axis and the electric potential (mV), adjusted with the associated palladium reference electrode, on the horizontal axis to provide ”quantity versus potential (QP) plot”. The patterns of the plots correspond to the derivative of a hydrodynamic voltammogram or a current-voltage curve, with the electric potentials of the peaks in the QP plot corresponding to the half-wave potentials in the current-voltage curve. We confirmed that catechins and theaflavins are oxidized depending on the electric potentials of their partial structures, and found that all compounds showing a peak at 0 mV in the QP plots produce hydrogen peroxide (H2O2) during the autoxidation process.
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ
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Ｊｏｕｒｎａｌ ｏｆ Ｃｌｉｎｉｃａｌ Ｂｉｏｃｈｅｍｉｓｔｒｙ ａｎｄ Ｎｕｔｒｉｔｉｏｎ 55 (2), 103-109, 2014
一般社団法人 日本酸化ストレス学会