<b>Determination of differential gene expression profiles in superficial and deeper zones of mature rat articular cartilage using RNA sequencing of laser microdissected tissue </b><b>specimens </b>
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- MORI Yoshifumi
- Sensory & Motor System Medicine, Faculty of Medicine, University of Tokyo
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- CHUNG Ung-il
- Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo
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- TANAKA Sakae
- Sensory & Motor System Medicine, Faculty of Medicine, University of Tokyo
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- SAITO Taku
- Sensory & Motor System Medicine, Faculty of Medicine, University of Tokyo Bone and CartilageRegenerative Medicine, Faculty of Medicine, University of Tokyo
Bibliographic Information
- Other Title
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- Determination of differential gene expression profiles in superficial and deeper zones of mature rat articular cartilage using RNA sequencing of laser microdissected tissue specimens
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Abstract
Superficial zone (SFZ) cells, which are morphologically and functionally distinct from chondrocytes in deeper zones, play important roles in the maintenance of articular cartilage. Here, we established an easy and reliable method for performance of laser microdissection (LMD) on cryosections of mature rat articular cartilage using an adhesive membrane. We further examined gene expression profiles in the SFZ and the deeper zones of articular cartilage by performing RNA sequencing (RNA-seq). We validated sample collection methods, RNA amplification and the RNA-seq data using real-time RT-PCR. The combined data provide comprehensive information regarding genes specifically expressed in the SFZ or deeper zones, as well as a useful protocol for expression analysis of microsamples of hard tissues.
Journal
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- Biomedical Research
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Biomedical Research 35 (4), 263-270, 2014
Biomedical Research Press