Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (<i>Brassica oleracea</i> subsp. <i>capitata</i>) “KY Cross”
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In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of <i>Brassica oleracea</i> ssp. <i>capitata</i> “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 µM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 µM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”.
- Plant Biotechnology
Plant Biotechnology 31(3), 275-280, 2014
Japanese Society for Plant Cell and Molecular Biology