Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (<i>Brassica oleracea</i> subsp. <i>capitata</i>) “KY Cross”

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著者

    • Ravanfar Ali Ravanfar Seyed Ali
    • Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia|Institute of Tropical Agriculture, Universiti Putra Malaysia
    • Abdullah Siti Nor Akmar
    • Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia|Institute of Tropical Agriculture, Universiti Putra Malaysia
    • Rashid Azmi Abdul
    • Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia

抄録

In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6- y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of <i>Brassica oleracea</i> ssp. <i>capitata</i> “KY Cross” were investigated. For hypocotyl explants, medium containing 2.27 µM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 µM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage “KY Cross”.

収録刊行物

  • Plant Biotechnology

    Plant Biotechnology 31(3), 275-280, 2014

    日本植物細胞分子生物学会

各種コード

  • NII論文ID(NAID)
    130004687377
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • ISSN
    1342-4580
  • NDL 記事登録ID
    025819066
  • NDL 請求記号
    Z54-J126
  • データ提供元
    NDL  J-STAGE 
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