<i>Dioscorea japonica</i> extract down-regulates prostaglandin E<sub>2</sub> synthetic pathway and induces apoptosis in lung cancer cells

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Abstract

Prostaglandin E<sub>2</sub> plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. Prostaglandin E<sub>2</sub> is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin E synthase. In some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin E synthase-1 are transiently induced, leading to prostaglandin E<sub>2</sub> overproduction. The present study showed that <i>Dioscorea japonica</i> extract suppresses mRNA expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in human non-small-cell lung carcinoma A549 cells in a dose-dependent manner. The suppressive effects of <i>Dioscorea japonica</i> extract on the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 were confirmed by Western blotting, cyclooxygenase activity and prostaglandin E<sub>2</sub> production. <i>Dioscorea japonica</i> extract induced the translocation of nuclear factor-κB from the nucleus to the cytosol and inhibited the activity of the cyclooxygenase-2 promoter. Furthermore <i>Dioscorea japonica</i> extract suppressed the expression of the anti-apoptotic factor B-cell chronic lymphocytic leukemia/lymphoma 2 and enhanced apoptotic terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive intensity in A549 cells. These results suggest that <i>Dioscorea japonica</i> extract suppresses the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1, with the regulation of the transcriptional activity of cyclooxygenase-2, and induces apoptosis in cancer cells. Thus, <i>Dioscorea japonica</i> may contribute to the prevention of prostaglandin E<sub>2</sub>-mediated pathophysiological responses such as carcinogenesis and inflammation.

Journal

  • Journal of Clinical Biochemistry and Nutrition

    Journal of Clinical Biochemistry and Nutrition 55(3), 162-167, 2014

    SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Codes

  • NII Article ID (NAID)
    130004687668
  • Text Lang
    ENG
  • ISSN
    0912-0009
  • Data Source
    J-STAGE 
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