Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules
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- Okazaki Yasumasa
- Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
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- Wang Yue
- Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
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- Tanaka Hiromasa
- Plasma Nanotechnology Research Center, Nagoya University Center for Advanced Medicine and Clinical Research, Nagoya University Hospital
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- Mizuno Masaaki
- Center for Advanced Medicine and Clinical Research, Nagoya University Hospital
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- Nakamura Kae
- Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
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- Kajiyama Hiroaki
- Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
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- Kano Hiroyuki
- NU Eco-Engineering Co. Ltd
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- Uchida Koji
- Laboratory of Food and Biodyamics, Graduate School of Bioagricultural Sciences, Nagoya University
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- Kikkawa Fumitaka
- Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine
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- Hori Masaru
- Plasma Nanotechnology Research Center, Nagoya University
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- Toyokuni Shinya
- Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine
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Abstract
Thermal plasmas and lasers are used in medicine to cut and ablate tissues and for coagulation. Non-equilibrium atmospheric pressure plasma (NEAPP) is a recently developed, non-thermal technique with possible biomedical applications. Although NEAPP reportedly generates reactive oxygen/nitrogen species, electrons, positive ions, and ultraviolet radiation, little research has been done into the use of this technique for conventional free radical biology. Recently, we developed a NEAPP device with high electron density. Electron spin resonance spin-trapping revealed •OH as a major product. To obtain evidence of NEAPP-induced oxidative modifications in biomolecules and standardize them, we evaluated lipid peroxidation and DNA modifications in various in vitro and ex vivo experiments. Conjugated dienes increased after exposure to linoleic and α-linolenic acids. An increase in 2-thiobarbituric acid-reactive substances was also observed after exposure to phosphatidylcholine, liposomes or liver homogenate. Direct exposure to rat liver in saline produced immunohistochemical evidence of 4-hydroxy-2-nonenal- and acrolein-modified proteins. Exposure to plasmid DNA induced dose-dependent single/double strand breaks and increased the amounts of 8-hydroxy-2'-deoxyguanosine and cyclobutane pyrimidine dimers. These results indicate that oxidative biomolecular damage by NEAPP is dose-dependent and thus can be controlled in a site-specific manner. Simultaneous oxidative and UV-specific DNA damage may be useful in cancer treatment.
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 55 (3), 207-215, 2014
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