Molecular Mechanisms of Membrane Proteins Studied by Infrared Spectroscopy
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- Furutani Yuji
- Institute for Molecular Science The Graduate University for Advanced Studies [SOKENDAI] JST PRESTO “Chemical conversion of light energy”
Bibliographic Information
- Other Title
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- 赤外分光法による膜タンパク質の分子機構研究
Abstract
Membrane proteins are indispensable for cell signaling, bioenergetics, and transportation. Atomic structures of membrane proteins have been elucidated by methods in structural biology (e.g. X-ray crystallography). Structural changes of them in action should be investigated to improve the understanding of the molecular mechanisms in more detail. Infrared spectroscopy is a powerful method to analyze structural changes of protein by combining it with various kinds of stimulus-induced difference spectroscopic techniques. In this review article, my recent studies on microbial rhodopsin, ion channel and ion pump proteins are summarized. Light-induced difference infrared spectroscopy revealed the hydrogen-bonding change of Thr204 in pharaonis phoborhodopsin upon photoisomerization of the retinal chromophore, which turned out to be a key residue for the signal transduction. Attenuated total reflection (ATR) method was applied on the studies of ion-protein interaction of a potassium channel, KcsA, and a sodium pump, V-type ATPase. Finally, development of a rapid-buffer exchange apparatus for time-resolved ATR infrared spectroscopy is introduced, which would be a powerful technique for investigating ion- and ligand-binding reactions of membrane proteins.
Journal
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- Molecular Science
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Molecular Science 8 (1), A0067-, 2014
Japan Society for Molecular Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001205282052992
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- NII Article ID
- 130004688035
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- ISSN
- 18818404
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed