Expression and regulation of Foxa2 in the rat uterus during early pregnancy

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  • YAMAGAMI Kazuki
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan
  • YAMAUCHI Nobuhiko
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan
  • KUBOTA Kaiyu
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan
  • NISHIMURA Sho
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan
  • CHOWDHURY Vishwajit Sur
    Division for Arts and Science, Faculty of Arts and Science, Kyushu University, Fukuoka 819-0395, Japan
  • YAMANAKA Kenichi
    Laboratory of Animal Resource Development, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
  • TAKAHASHI Masashi
    Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
  • TABATA Shoji
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan
  • HATTORI Masa-aki
    Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka 812-8581, Japan

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Abstract

The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly understood. Therefore, the present study was conducted to investigate the expressional profiles of Foxa2 in the rat uterus during the estrus cycle and pregnancy. Furthermore, the effect of steroid hormones and Hedgehog protein on the expression of Foxa2 was analyzed in vivo and in vitro. In this study, the level of expression of Foxa2 was low in the rat uterus during the different stages of the estrus cycle. However, the expression increased transiently during early pregnancy at 3.5 days post coitus (dpc) and decreased at 5.5 dpc. In ovariectomized rats, P4 treatment had no effect on the expression of Foxa2 compared with the expression in control animals. Moreover, the expression of Foxa2 in cultured epithelial cells was not increased by P4 treatment in vitro. However, Foxa2 expression was significantly decreased in the rat uterus after 24 h of E2 treatment. Treatment of cells with a recombinant Hedgehog protein significantly increased the expression of Foxa2. These results suggest that the expression of Foxa2 may transiently increase just before the implantation and it may be regulated by E2 and Hedgehog protein.

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