Bacteria of the Candidate Phylum TM7 are Prevalent in Acidophilic Nitrifying Sequencing-Batch Reactors

• Hanada Akiko

  Department of Environmental and Life Sciences, Toyohashi University of Technology

• Kurogi Takashi

  Department of Environmental and Life Sciences, Toyohashi University of Technology

• Giang Nguyen Minh

  Department of Environmental and Life Sciences, Toyohashi University of Technology

• Yamada Takeshi

  Department of Environmental and Life Sciences, Toyohashi University of Technology

• Kamimoto Yuki

  EcoTopia Science Institute, Nagoya University

• Kiso Yoshiaki

  Department of Environmental and Life Sciences, Toyohashi University of Technology

• Hiraishi Akira

  Department of Environmental and Life Sciences, Toyohashi University of Technology

抄録

Laboratory-scale acidophilic nitrifying sequencing-batch reactors (ANSBRs) were constructed by seeding with sewage-activated sludge and cultivating with ammonium-containing acidic mineral medium (pH 4.0) with or without a trace amount of yeast extract. In every batch cycle, the pH varied between 2.7 and 4.0, and ammonium was completely converted to nitrate. Attempts to detect nitrifying functional genes in the fully acclimated ANSBRs by PCR with previously designed primers mostly gave negative results. 16S rRNA gene-targeted PCR and a subsequent denaturating gradient gel electrophoresis analysis revealed that a marked change occurred in the bacterial community during the overall period of operation, in which members of the candidate phylum TM7 and the class Gammaproteobacteria became predominant at the fully acclimated stage. This result was fully supported by a 16S rRNA gene clone library analysis, as the major phylogenetic groups of clones detected (>5% of the total) were TM7 (33%), Gammaproteobacteria (37%), Actinobacteria (10%), and Alphaproteobacteria (8%). Fluorescence in situ hybridization with specific probes also demonstrated the prevalence of TM7 bacteria and Gammaproteobacteria. These results suggest that previously unknown nitrifying microorganisms may play a major role in ANSBRs; however, the ecophysiological significance of the TM7 bacteria predominating in this process remains unclear.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 29 (4), 353-362, 2014

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• acidophilic nitrification
• ammonia oxidation
• TM7 bacteria
• <i>amoA</i> gene

詳細情報

• CRID: 1390282679323117312
• NII論文ID: 40020304032
• NII論文ID: 130004690769
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me14052
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 025987327
• PubMed: 25241805
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025987327
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/29/4/29_ME14052/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可