Down-regulation of cyclooxygenase-2 (COX-2) by cannabidiolic acid in human breast cancer cells

• Takeda Shuso

  Department of Molecular Biology, Daiichi University of Pharmacy Laboratory of Xenobiotic Metabolism and Environmental Toxicology, Faculty of Pharmaceutical Sciences,　Hiroshima International University (HIU)

• Okazaki Hiroyuki

  Drug Innovation Research Center, Daiichi University of Pharmacy

• Ikeda Eriko

  Department of Molecular Biology, Daiichi University of Pharmacy

• Abe Satomi

  Department of Molecular Biology, Daiichi University of Pharmacy

• Yoshioka Yasushi

  Department of Molecular Biology, Daiichi University of Pharmacy

• Watanabe Kazuhito

  Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Aramaki Hironori

  Department of Molecular Biology, Daiichi University of Pharmacy Drug Innovation Research Center, Daiichi University of Pharmacy

Bibliographic Information

Other Title

• Toxicomics Report : Down-regulation of cyclooxygenase-2 (COX-2) by cannabidiolic acid in human breast cancer cells

Abstract

Metastases are known to be responsible for approximately 90% of breast cancer-related deaths. Cyclooxygenase-2 (COX-2) is involved not only in inflammatory processes, but also in the metastasis of cancer cells; it is expressed in 40% of human invasive breast cancers. To comprehensively analyze the effects of cannabidiolic acid (CBDA), a selective COX-2 inhibitor found in the fiber-type cannabis plant (Takeda et al., 2008), on COX-2 expression and the genes involved in metastasis, we performed a DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are invasive breast cancer cells that express high levels of COX-2, treated with CBDA for 48 hr at 25 µM. The results obtained revealed that COX-2 and Id-1, a positive regulator of breast cancer metastasis, were down-regulated (0.19-fold and 0.52-fold, respectively), while SHARP1 (or BHLHE41), a suppressor of breast cancer metastasis, was up-regulated (1.72-fold) and CHIP (or STUB1) was unaffected (1.03-fold). These changes were confirmed by real-time RT-PCR analyses. Taken together, the results obtained here demonstrated that i) CBDA had dual inhibitory effects on COX-2 through down-regulation and enzyme inhibition, and ii) CBDA may possess the ability to suppress genes that are positively involved in the metastasis of cancer cells in vitro.

Journal

• The Journal of Toxicological Sciences

  The Journal of Toxicological Sciences 39 (5), 711-716, 2014

  The Japanese Society of Toxicology

Keywords

• Cannabidiolic acid
• Cyclooxygenase-2
• Human breast cancer cells
• Fiber-type cannabis plant
• Metastasis

Details

• CRID: 1390282679882502656
• NII Article ID: 130004690957
• NII Book ID: AN00002808
• DOI: 10.2131/jts.39.711
• COI: 1:STN:280:DC%2BC2M7jsFOiug%3D%3D
• ISSN: 18803989
• ISSN: 03881350
• NDL BIB ID: 025850822
• PubMed: 25242400
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025850822
• Web Site: https://www.jstage.jst.go.jp/article/jts/39/5/39_711/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015134681
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed