Comparative metabolome analysis of cultured fetal and adult hepatocytes in humans

• Kim Su-Ryang

  Division of Pharmacology, National Institute of Health Sciences

• Kubo Takashi

  Division of Pharmacology, National Institute of Health Sciences Present address: Division of Translational Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center

• Kuroda Yukie

  Division of Pharmacology, National Institute of Health Sciences

• Hojyo Maki

  Division of Pharmacology, National Institute of Health Sciences

• Matsuo Takuya

  Faculty of Biotechnology and Life Science, Sojo University

• Miyajima Atsuko

  Division of Medical Devices, National Institute of Health Sciences

• Usami Makoto

  Division of Pharmacology, National Institute of Health Sciences

• Sekino Yuko

  Division of Pharmacology, National Institute of Health Sciences

• Matsushita Taku

  Faculty of Biotechnology and Life Science, Sojo University

• Ishida Seiichi

  Division of Pharmacology, National Institute of Health Sciences

Abstract

The liver is the central organ of metabolism, but its function varies during development from fetus to adult. In this study, we comprehensively analyzed and compared metabolites in fetal and adult hepatocytes, the major parenchymal cell in the liver, from human donors. We identified 211 metabolites (116 anions and 95 cations) by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) in the hepatocytes cultured in vitro. Principal component analysis and hierarchical clustering analysis of the relative amounts of metabolites clearly classified hepatocytes into 2 groups that were consistent with their origin, i.e., the fetus and adult. The amounts of most metabolites in the glycolysis/glyconeogenesis pathway, tricarboxylic acid cycle and urea cycle were lower in fetal hepatocytes than in adult hepatocytes. These results suggest different susceptibility of the fetal and adult liver to toxic insults affecting energy metabolism.

Journal

• The Journal of Toxicological Sciences

  The Journal of Toxicological Sciences 39 (5), 717-723, 2014

  The Japanese Society of Toxicology

Keywords

• Metabolome
• CE-TOFMS
• Human fetal hepatocytes
• Human adult hepatocytes

Details

• CRID: 1390001204905803648
• NII Article ID: 130004690958
• NII Book ID: AN00002808
• DOI: 10.2131/jts.39.717
• COI: 1:STN:280:DC%2BC2M7jsFOiuw%3D%3D
• ISSN: 18803989
• ISSN: 03881350
• NDL BIB ID: 025850840
• PubMed: 25242401
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025850840
• Web Site: https://www.jstage.jst.go.jp/article/jts/39/5/39_717/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015134682
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed