Sensitive and Rapid Detection of <i>Campylobacter</i> Species from Stools of Children with Diarrhea in Japan by the Loop-Mediated Isothermal Amplification Method

• Ushijima Hiroshi

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Nishimura Shuichi

  Nishimura Clinic

• Thongprachum Aksara

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Shimizu-Onda Yuko

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine

• Tran Dinh Nguyen

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Pham Ngan Thi Kim

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine

• Takanashi Sayaka

  Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Dey Shuvra Kanti

  Department of Microbiology, Jahanghirnagar University

• Okitsu Shoko

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Yamazaki Wataru

  Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki

• Mizuguchi Masashi

  Department of Developmental Medical Sciences, Graduate School of Medicine, the University of Tokyo

• Hayakawa Satoshi

  Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine

Bibliographic Information

Other Title

• Sensitive and Rapid Detection of Campylobacter Species from Stools of Children with Diarrhea in Japan by the Loop-Mediated Isothermal Amplification Method
• Sensitive and rapid detection of Campylobacter species from stools of diarrheal children in Japan by LAMP method

Abstract

We detected Campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in Kyoto using a commercial loop-mediated isothermal amplification (LAMP) kit with a fluorescent detection reagent after DNA extraction. The sensitivity and specificity were 100% in comparison with those of semi-nested PCR for the differentiation of Campylobacter jejuni and Campylobacter coli. Fourteen of the 20 samples were already determined as C. jejuni by the culture method. All 20 samples were also positive for C. jejuni by the PCR method. Among the 58 cultured samples, the sensitivity of the culture method against the LAMP method was 93.3% (14/15) and the specificity was 100% (43/43). The detection rate of Campylobacter spp. from the heated supernatants by the LAMP method was lower than that from the supernatant after DNA extraction. In total, 25% (5/20) of the Campylobacter-positive samples by the LAMP method were co-infected with norovirus (3/20), rotavirus (1/20), and human parechovirus (1/20), although no other bacterial co-infection was identified by the culture method. C. jejuni was mostly detected in children aged >5 years throughout the year. Based on these results, we concluded that care should be taken while diagnosing Campylobacter infection in children. Our newly modified LAMP method is a rapid, easy, and useful method for this diagnosis.

Journal

• Japanese Journal of Infectious Diseases

  Japanese Journal of Infectious Diseases 67 (5), 374-378, 2014

  National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Keywords

• rapid detection
• LAMP
• PCR
• culture method
• <i>Campylobacter</i>

Details

• CRID: 1390282681216406528
• NII Article ID: 130004692237
• NII Book ID: AA1132885X
• DOI: 10.7883/yoken.67.374
• ISSN: 18842836
• ISSN: 13446304
• NDL BIB ID: 025805280
• PubMed: 25241688
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025805280
• Web Site: https://search.jamas.or.jp/link/ui/2015307953
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed