Amphotericin B Induces Glial Cell Line-Derived Neurotrophic Factor in the Rat　Brain

• MOTOYOSHI-YAMASHIRO Akiko

  Laboratory of Integrative Physiology in Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• TAKANO Katsura

  Laboratory of Integrative Physiology in Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• KAWABE Kenji

  Laboratory of Integrative Physiology in Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• IZAWA Takeshi

  Laboratory of Veterinary Pathology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• NAKAJIMA Hidemitsu

  Laboratory of Veterinary Pharmacology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• MORIYAMA Mitsuaki

  Laboratory of Integrative Physiology in Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

• NAKAMURA Yoichi

  Laboratory of Integrative Physiology in Veterinary Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan

Bibliographic Information

Other Title

• Physiology : Amphotericin B Induces Glial Cell Line-Derived Neurotrophic Factor in the Rat Brain

Abstract

Amphotericin B (AmB) is a　polyene antifungal drug and is reported to be one of a few reagents having therapeutic　effects on prion diseases, that is, a delay in the appearance of clinical signs and　prolongation of the survival time in an animal model. In prion diseases, glial cells have　been suggested to play important roles; however, the therapeutic mechanism of AmB on prion diseases remains elusive. We have previously reported that AmB changed the expression of neurotrophic factors in microglia and astrocytes (Motoyoshi et al., 2008, Neurochem. Int. 52, 1290–1296; Motoyoshi-Yamashiro et al., 2013, ibid. 63, 93–100). These results suggested that neurotrophic factors derived from glial cells might be involved in the therapeutic mechanism of AmB. In the present study, we examined immunohistochemically the effects of AmB on the expression of neurotrophic factors in the rat brain. We found that direct injection of AmB into the striatum significantly enhanced the expression of glial cell line-derived neurotrophic factor protein. Amphotericin B also increased the expressions of CD11b and glial fibrillary acidic protein, markers of microglia and astrocytes, respectively. Moreover, expressions of the two neurotrophic factors by AmB were co-localized with the expression of CD11b or glial fibrillary acidic protein. These results suggest that AmB in vivo might also activate glial cells and induce the production of neurotrophic factors protecting neurons in prion diseases.

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 76 (10), 1353-1358, 2014

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• amphotericin B
• astrocytes
• microglia
• neurotrophic factors

Details

• CRID: 1390282681407524352
• NII Article ID: 130004693905
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.14-0160
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 025876043
• PubMed: 25283947
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025876043
• Web Site: https://www.jstage.jst.go.jp/article/jvms/76/10/76_14-0160/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015084926
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed