Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomal RNA among three <i>Moniezia</i> species isolated from ruminants in Japan
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- OHTORI Maiko
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
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- AOKI Mikiko
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
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- ITAGAKI Tadashi
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3–18–8, Morioka 020–8550, Japan
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Abstract
This study was designed to clarify the differences in the internal transcribed spacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa, M. benedeni and M. monardi isolated from ruminants in Japan and to determine their phylogenetic relationships. A 98% similarity in the 5.8S sequences was observed among the 3 Moniezia species, whereas many nucleotide indels and substitutions were observed in the ITS1 sequences among the three Moniezia species. These results suggest that the ITS1 region could serve as a potential marker for discriminating the 3 Moniezia species. In the phylogenetic tree based on the ITS1 sequences, M. monardi and M. benedeni showed genetically closer relationship to each other than to M. expansa.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 77 (1), 105-107, 2015
JAPANESE SOCIETY OF VETERINARY SCIENCE
