Interfacial Behavior of Pulmonary Surfactant Preparations Containing Egg Yolk Lecithin

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  • Nakahara Hiromichi
    Department of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Nagasaki International University
  • Shibata Osamu
    Department of Biophysical Chemistry, Graduate School of Pharmaceutical Sciences, Nagasaki International University

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Mammalian lungs are covered with lipid-protein complexes or pulmonary surfactants. In this work, which aimed towards the less expensive production of artificial pulmonary surfactants, we produced surfactants composed of egg yolk lecithin (eggPC), palmitic acid, and hexadecanol (= 0.30/0.35/0.35, mol/mol/mol ) containing different amounts of Hel 13-5 (NH2-KLLKLLLKLWLKLLKLLL-COOH) as a substitute for the proteins in native pulmonary surfactants. Surface pressure (π)–molecular area (A) and surface potential (DV)–A isotherms of the mixtures were measured via the Wilhelmy and ionizing 241Am electrode methods, respectively. The interactions between the lipid components and Hel 13-5 led to variations in the surface pressure caused by the expulsion of fluid components from the surface. Furthermore, the π–A and DV–A isotherms featured large hysteresis loops for the surfactant that contained a small amount of Hel 13-5 during compression and successive expansion cycling. To elucidate the morphology, the phase behavior was visualized in situ at the air–water interface by means of fluorescence microscopy; the images suggested less effective interactions between Hel 13-5 and the unsaturated PC in eggPC despite the similarity of their monolayer properties.

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