Analysis of chemokine production at TNF-<i>α</i>-stimulated synovial cells from human TMJ by antibody array

DOI
  • AKUTSU Miwa
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • KAWASHIMA Mutsumi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • OGURA Naomi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • HATTORI Toshio
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • YAMAZAKI Fumie
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • ITO Ko
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • KONDOH Toshirou
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

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  • 抗体アレイを用いたTNF-<i>α</i>刺激ヒト顎関節滑膜細胞のケモカイン産生解析

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Abstract

Tumor necrosis factor-α (TNF-α) is a key inflammatory mediator in the temporomandibular joint (TMJ) exhibiting internal derangement. In this study, an antibody array was used to study the TNF-α-stimulated protein production profile of synovial fibroblasts. Synovial fibroblasts were prepared using the outgrowth method from the tissues of patients suffering from internal derangement of the TMJ. The synovial cells were stimulated by TNF-α for 4 hours, and the release of chemokines into the supernatant was determined using a human chemokine antibody array. The result of the antibody array indicated that TNF-α had a net stimulatory effect on chemokine levels. A total of seven chemokines were significantly upregulated, and the expression of these genes was analysed by a real-time polymerase chain reaction. Following stimulation with TNF-α for 2, 4, or 8 hours, expression of the seven chemokine genes was upregulated compared with untreated controls. These results suggest that TNF-α-induced chemokine production may be responsible for the inflammation associated with internal derangement of the TMJ.

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