Regulatory/Effector T-Cell Ratio Is Reduced in Coronary Artery Disease

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著者

    • Emoto Takuo Emoto Takuo
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Sasaki Naoto Sasaki Naoto
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Kasahara Kazuyuki
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Yodoi Keiko
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Sasaki Yoshihiro
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Matsumoto Takuya
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Mizoguchi Taiji
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Hirata Ken-ichi
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine

抄録

<b><i>Background:</i></b>The protective function of regulatory T cells (T<sub>reg</sub>) has been identified in experimental atherosclerosis, but the contribution of T<sub>reg</sub>to the pathogenesis of human coronary artery disease (CAD) remains poorly understood. We investigated T<sub>reg</sub>and regulatory T-cell/effector T-cell (T<sub>reg</sub>/T<sub>eff</sub>) ratio in peripheral blood samples from CAD patients using a new strategy for precise identification of T<sub>reg</sub>.<b><i>Methods and Results:</i></b>Peripheral blood samples were collected from 73 stable CAD patients (55 middle-aged CAD patients and 18 old CAD patients) and 64 controls (47 middle-aged controls and 17 young controls). CD3<sup>+</sup>CD4<sup>+</sup>FoxP3<sup>+</sup>T cells were divided into 3 fractions: CD45RA<sup>+</sup>FoxP3<sup>low</sup>resting T<sub>reg</sub>(Fr1), CD45RA<sup>–</sup>FoxP3<sup>high</sup>activated T<sub>reg</sub>(Fr2), and CD45RA<sup>–</sup>FoxP3<sup>low</sup>non-T<sub>reg</sub>(Fr3). CAD patients had lower percentages of Fr1 and Fr2 and higher percentages of Fr3 and CD45RA<sup>–</sup>Foxp3<sup>–</sup>T<sub>eff</sub>(Fr4+5) within the CD3<sup>+</sup>CD4<sup>+</sup>T-cell population compared to age-matched controls. T<sub>reg</sub>/T<sub>eff</sub>ratio (Fr1+2/Fr3+4+5) in CAD patients was also markedly lower than in controls (middle-aged control, 0.17±0.09 vs. middle-aged CAD, 0.10±0.05; P<0.001). The percentage of CD4<sup>+</sup>CD28<sup>null</sup>T cells within the CD4<sup>+</sup>T-cell population was negatively correlated with T<sub>reg</sub>/T<sub>eff</sub>ratio, excluding CD4<sup>+</sup>CD28<sup>null</sup>T cells <0.3% (r=–0.27, P<0.05). High-sensitivity C-reactive protein was also negatively correlated with T<sub>reg</sub>/T<sub>eff</sub>ratio (r=–0.22, P<0.05).<b><i>Conclusions:</i></b>CAD patients had reduced T<sub>reg</sub>and T<sub>reg</sub>/T<sub>eff</sub>ratio compared to healthy controls. The present findings may be helpful when developing immunotherapy for the prevention of CAD. (<i>Circ J</i> 2014; <b>78:</b> 2935–2941)

収録刊行物

  • 日本循環器學誌

    日本循環器學誌 78(12), 2935-2941, 2014

    一般社団法人 日本循環器学会

各種コード

  • NII論文ID(NAID)
    130004699205
  • NII書誌ID(NCID)
    AA11591968
  • 本文言語コード
    ENG
  • ISSN
    1346-9843
  • NDL 記事登録ID
    025928516
  • NDL 請求記号
    Z54-B860
  • データ提供元
    NDL  J-STAGE 
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