SRD Outstanding Research Award 2013 : Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle

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  • AKAGI Satoshi
    Animal Breeding and Reproduction Research Division, NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • MATSUKAWA Kazutsugu
    Multidisciplinary Science Cluster, Life and Environmental Medicine Science Unit, Kochi University, Kochi 783-8502, Japan
  • TAKAHASHI Seiya
    Headquarters, National Agriculture and Food Research Organization, Ibaraki 305-8517, Japan

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  • Factors Affecting the Development of Somatic Cell Nuclear Transfer Embryos in Cattle

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Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor cells, enucleation, cell fusion, oocyte activation and embryo culture. Therefore, many factors are believed to contribute to the success of embryo development following nuclear transfer. Numerous attempts to improve cloning efficiency have been conducted since the birth of the first sheep by somatic cell nuclear transfer. However, the efficiency of somatic cell cloning has remained low, and applications have been limited. In this review, we discuss some of the factors that affect the developmental ability of somatic cell nuclear transfer embryos in cattle.

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